Effects of peritoneal dialysis solution on apoptosis and intracellular free calcium, cell surface ICAM-1 expression of rat peritoneal mesothelial cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1752-1754, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-282614
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of peritoneal dialysis solution (PDS) on apoptosis and intracellular free calcium([Ca(2+)]i), cell surface ICAM-1 expression of rat peritoneal mesothelial cells (RPMCs).</p><p><b>METHODS</b>The RPMCs apoptosis rate were determined by flow cytometry. [Ca(2+)]i in the cells were monitered the fluorescence at 528 nm by confocus laser microscopy. Cell surface ICAM-1 expression were detected by flow cytometry.</p><p><b>RESULT</b>After PDS treatment for 1 h, the RPMCs apoptosis rate were increased. Such increase was more manifest with higher glucose concentration in PDS and longer treatment time of the cells. At the same times, after 3 hours, ICAM-1 expressions of the PDS containing glucose and mannitol are all increased. With the increase of glucose concentrations, the descend of [Ca(2+)]i levels were aggravated.</p><p><b>CONCLUSION</b>PDS containing high- concentration glucose can induce significant apoptosis of RPMCs in vitro. This may be related with the enhanced level of ICAM-1 expressions and the decreased level of [Ca(2+)]i. Which may due to the occurrence of peritoneal fibrosis and ultrafiltrate failure in patients suffering long term peritoneal dialysis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peritoneal Cavity
/
Pharmacology
/
Dialysis Solutions
/
Gene Expression Regulation
/
Calcium
/
Peritoneal Dialysis
/
Rats, Sprague-Dawley
/
Apoptosis
/
Intercellular Adhesion Molecule-1
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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