Expression of B7-related protein-1 on human coronary artery endothelial cells and its clinical significance / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1623-1625, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-282632
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of oxidized low density lipoprotein (ox-LDL) on the expression of B7-related protein-1 (B7RP-1) on human coronary artery endothelial cells (HCAECs).</p><p><b>METHODS</b>HCAECs were incubated in the presence of 100 mg/L ox-LDL for 24 h, and B7RP-1 expression levels were determined using fluorescence reverse transcription PCR (RT-PCR) and Western blotting.</p><p><b>RESULTS</b>B7RP-1 expression was detected HCAECs, with spots of fluorescence signals distributing on the cell membrane as observed under fluorescence microscope. RT-PCR with B7RP-1 specific primers yielded products of expected size (496 bp). Western blotting identified B7RP-1 expression in the HCAECs as a cell-associated protein with an apparent molecular mass of 70,000. Treatment of the cells with ox-LDL significantly increased B7RP-1 expression at both the mRNA and protein levels (P<0.05).</p><p><b>CONCLUSION</b>B7RP-1 is expressed on the membrane of HCAECs. ox-LDL can promote up-regulate the expression of B7RP-1, which might be one of the immunopathogenesis of atherosclerosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Cell Line
/
Gene Expression Regulation
/
B7-1 Antigen
/
Coronary Vessels
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Endothelial Cells
/
Inducible T-Cell Co-Stimulator Ligand
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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