Effect of protein kinase CK2 gene silencing on radiosensitization in human nasopharyngeal carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1551-1553, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-282654
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of protein kinase CK2 gene silencing on the radiosensitization in human nasopharyngeal carcinoma (NPC) cells and its possible mechanism.</p><p><b>METHODS</b>RNA interference (RNAi) technique was used to down-regulate the protein kinase CK2alpha expression in 5-8F cells, and clonogenic assay was employed to observe the changes in the radiosensitivity of the cells. DNA double-strand break was assessed by immunofluorescence staining of gamma-H2AX foci, and the cell apoptosis was examined using Annexin V-FITC/PI double-staining flow cytometry.</p><p><b>RESULTS</b>CK2alpha protein was successfully silenced by siRNA. CK2alpha knockdown significantly decreased the clonogenic activity and increased the radiosensitivity of the NPC cells. After a 15-min exposure of the cells to 1 Gy radiation, significant difference occurred in the gamma-H2AX foci between CK2alpha knockdown cells and the control cells (P<0.01). CK2alpha silencing significantly increased the cell apoptosis after the exposure (P<0.01).</p><p><b>CONCLUSIONS</b>Protein kinase CK2 plays an important role in the radiosensitivity of the NPC cells, and suppression of its expression might be a potential therapeutic approach of cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Radiation Tolerance
/
Histones
/
Transfection
/
Nasopharyngeal Neoplasms
/
Annexin A5
/
RNA, Small Interfering
/
RNA Interference
/
Cell Line, Tumor
/
Casein Kinase II
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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