Effect of ClpE depletion on the bacterial protein expression profiles of Streptococcus pneumoniae / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1533-1537, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-282659
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of ClpE on the protein expression profiles of Streptococcus pneumoniae.</p><p><b>METHODS</b>clpE-deficient Streptococcus pneumoniae strain was constructed by long flanking homology-polymerase chain reaction (LFH-PCR) and identified by PCR and sequencing. The total bacterial proteins were analyzed by two-dimensional gel electrophoresis and imaging analysis, and the differentially expressed protein spots were excised by dot-gel digestion with trypsin. Peptide mass fingerprinting (PMF) was obtained by analysis of the fragment length by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The PMF was analyzed using software to identify the proteins.</p><p><b>RESULTS</b>The number of matched protein spots of the two gels was 61%. By sequence database searching, 4 out of the 17 differential protein spots were identified, namely hypoxanthine-guanine, pyrrolidone-carboxylate peptidase1, formate-tetrahydrofolate ligase, and bifunctional protein pyrR.</p><p><b>CONCLUSION</b>clpE gene-deficient Streptococcus pneumoniae expresses fewer kinds of proteins at also lower levels than the wild-type bacteria, suggesting that ClpE allows the bacteria to adapt to different host environments by inducing the expression of special proteins.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Streptococcus pneumoniae
/
Bacterial Proteins
/
Electrophoresis, Gel, Two-Dimensional
/
Polymerase Chain Reaction
/
Adenosine Triphosphatases
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Gene Expression Profiling
/
Genetics
/
Heat-Shock Proteins
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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