RHD 1227A allele frequency among Rh negative population and random population / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1234-1237, 2006.
Article
in Zh
| WPRIM
| ID: wpr-282693
Responsible library:
WPRO
ABSTRACT
To investigate the frequency of RHD 1227A allele in Rh negative population and random population, an AS-PCR (allele specific-polymerase chain reaction) method was employed to detect RHD 1227A allele. RHD gene copy was determined by D zygosity test and RHD exon 9 nucleotide sequence analysis. The results showed that among 143 Rh negative donors, forty-one RHD 1227A allele carriers were detected, and 8 (19.51%) out of which were RhCCdee, 32 (78.05%) were RhCcdee, and 1 (2.44%) was RhCcdEe. Thirty-five Rh negative RHD 1227A carriers had RHD gene deletion, and the remaining carriers were RHD 1227A homozygous. Seven (1.43%) individuals were detected with RHD 1227A allele among 489 random donors. They were all G/A heterozygous at RHD 1227 site. Serological test indicated that they were normal Rh positive phenotype. It is concluded that the frequency of RHD 1227A allele is 16.43% among Rh negative population and 0.72% among the random population.
Full text:
1
Index:
WPRIM
Main subject:
Polymorphism, Genetic
/
Rh-Hr Blood-Group System
/
Molecular Sequence Data
/
Base Sequence
/
China
/
Chromosome Deletion
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
Gene Deletion
/
Asian People
Type of study:
Clinical_trials
Limits:
Humans
Country/Region as subject:
Asia
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article