Feasibility of HLA-DRB1 matching by using DHPLC / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1183-1187, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-282704
ABSTRACT
To study feasibility of HLA-DRB1 matching by using denatured high performance liquid chromatography (DHPLC), 20 pairs of DNA samples from donors and recipients of hematopoietic cell transplantation (HCT) for DRB1 matching and 2 pairs of samples from donors and recipient of HCT for DRB1 mismatching were studied by DHPLC and PCR-SSP. After being amplified and annealed slowly to produce heteroduplex, PCR products for exon 2 of DRB1 were detected by DHPLC to find matched or mismatched peaks in chromatogram. The results showed that DHPLC and PCR-SSP were consistant with matched or mismatched HLA-DRB1 typing. The results of DHPLC and PCR-SSP for matching were compared by using kappa test (kappa = 0.776, P = 0.00), which suggested DHPLC for HLA-DRB1 matching was in agreement with PCR-SSP. In conclusion, DHPLC for HLA-DRB1 matching is economic and convenient, moreover, will not be affected by unknown genes in HLA-DRB1 locus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Blood Donors
/
Histocompatibility Testing
/
Molecular Sequence Data
/
Base Sequence
/
HLA-DR Antigens
/
Feasibility Studies
/
Chromatography, High Pressure Liquid
/
Hematopoietic Stem Cell Transplantation
/
Allergy and Immunology
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article
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