Construction of HA-1-DC nucleic-acid vaccine and induction of specific cytotoxic T lymphocytes / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1178-1182, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-282705
ABSTRACT
The purpose of this study was to construct a HA-1-DC nucleic acid vaccine and to induce anti-leukemia effect after hematopoietic stem cell transplantation (HSCT). The dendritic cells (DCs) were generated from HSCT donors in vitro, and its immunologic activity was studied by using flow cytometry and mix lymphocyte reaction. HA-1 gene was electroporated into the cultured DCs to construct a DC nucleic acid vaccine. After transfecting for 48 hours, the expression of HA-1 protein was detected by Western blot. The DCs were cultured with isogenic lymphocytes to induce specific cytotoxic T lymphocytes (CTLs). The cytotoxicity of the CTLs was detected by LDH assay. The results showed that the DCs derived from peripheral blood monocytes (PBMCs) expressed the DC phenotype, and were effective in stimulating proliferation of the allogenic lymphocytes. After electroporating for 48 hours, HA-1 protein was detected by Western blot. The cytotoxity of inducing CTLs was higher than that in the control group. It is concluded that the minor histocompatibility antigen HA-1 can be considered as a target of immunotherapy against leukemia after HSCT.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligopeptides
/
Therapeutics
/
Dendritic Cells
/
Transfection
/
T-Lymphocytes, Cytotoxic
/
Leukemia
/
Minor Histocompatibility Antigens
/
Cells, Cultured
/
Electroporation
/
Hematopoietic Stem Cell Transplantation
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article
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