Production of specific CTL induced by exosomes derived from K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1168-1171, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-282707
ABSTRACT
The aim of this study was to investigate whether exosomes derived from K562 cells and human monocyte-derived dendritic cells (DCs) transfected with total RNA of K562 cells are capable of inducing antigen-specific cytotoxic T lymphocytes (CTL) responses in vitro. DCs were generated from peripheral blood mononuclear cells (PBMNC) of healthy volunteers in the presence of GM-CSF and IL-4, and then were transfected with K562 RNA by using DOTAP lipofection. Exosomes was extracted from the supernatant of DCs and K562 cells. The T cell were activated to be tumor specific CTL after DCs and exosomes were co-cultured with autologous T cells derived from healthy volunteers' PBMNC. The effect of CTL on K562 cells was detected by MTT assay. The results showed that treatment of T cells with exosomes derived from K562 cells or DCs transfected with total RNA of K562 cells could significantly promote their killing ability on K562 cells as compared with untreated T cells (P < 0.05). The killing ability of T cells treated with exosomes on K562 cells was stronger than on HL-60 cells (P < 0.05). It is concluded that the specific CTL immune response to leukemia cells can be induced by exosomes derived from K562 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Endosomes
/
Dendritic Cells
/
RNA, Neoplasm
/
Monocytes
/
Transfection
/
T-Lymphocytes, Cytotoxic
/
K562 Cells
/
Cell Biology
/
Allergy and Immunology
/
Exocytosis
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article
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