GATA-3 expression in the development of anterior chamber associated immune deviation / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2000-2004, 2005.
Article
in English
| WPRIM
| ID: wpr-282832
ABSTRACT
<p><b>BACKGROUND</b>Anterior chamber associated immune deviation (ACAID) is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID.</p><p><b>METHODS</b>ACAID was induced by injection of 50 microg interphotoreceptor retinoid binding protein (IRBP) into the anterior chamber (AC) of Wistar rats. Delayed-type hypersensitivity (DTH) was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>A significant DTH reaction was observed in Wistar rats on day 3 and 5 after IRBP inoculation. The DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP.</p><p><b>CONCLUSION</b>The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Spleen
/
Retinol-Binding Proteins
/
RNA, Messenger
/
Rats, Wistar
/
Th2 Cells
/
Allergy and Immunology
/
GATA3 Transcription Factor
/
Eye Proteins
/
Genetics
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2005
Type:
Article
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