Altered expression of renal bumetanide-sensitive sodium-potassium-2 chloride cotransporter and Cl- channel -K2 gene in angiotensin II-infused hypertensive rats / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1945-1951, 2005.
Article
in English
| WPRIM
| ID: wpr-282840
ABSTRACT
<p><b>BACKGROUND</b>Little information is available regarding the effect of angiotensin II (Ang II) on the bumetanide-sensitive sodium-potassium-2 chloride cotransporter (NKCC2), the thiazide-sensitive sodium-chloride cotransporter (NCC), and the Cl- channel (CLC)-K2 at both mRNA and protein expression level in Ang II-induced hypertensive rats. This study was conducted to investigate the influence of Ang II with chronic subpressor infusion on nephron-specific gene expression of NKCC2, NCC and CLC-K2.</p><p><b>METHODS</b>Sprague Dawleys rats were treated subcutaneously with either Ang II (100 ng.kg-1.min-1) or vehicle for 14 days. Expression of NKCC2, NCC and CLC-K2 mRNA in kidneys was determined by real time polymerase chain reaction (PCR). Western blotting analysis was used to measure NKCC2 and NCC protein expression.</p><p><b>RESULTS</b>Ang II significantly increased blood pressure and up-regulated NKCC2 mRNA and protein expression in the kidney. Expression of CLC-K2 mRNA in the kidney increased 1.6 fold (P < 0.05). There were no changes in NCC mRNA or protein expression in AngII-treated rats versus control.</p><p><b>CONCLUSIONS</b>Chronic subpressor Ang II infusion can significantly alter NKCC2 and CLC-K2 mRNA expression in the kidney, and protein abundance of NKCC2 in kidney is positively regulated by Ang II. These effects may contribute to enhanced renal Na+ and Cl- reabsorption in response to Ang II.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Blood Pressure
/
RNA, Messenger
/
Angiotensin II
/
Gene Expression Regulation
/
Rats, Sprague-Dawley
/
Sodium-Potassium-Chloride Symporters
/
Solute Carrier Family 12, Member 1
/
Genetics
/
Hypertension
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2005
Type:
Article
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