Screening, isolation and identification of nisin resistance determinant gene in strains of Lactococcus lactis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 839-842, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-282902
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and identify nisin resistance determinant (NSR) gene from Lactococcus lactis.</p><p><b>METHODS</b>The Lactococcus lactis strains harboring NSR gene were isolated from different milk samples by selective culture supplemented with nisin and confirmed by PCR detection of 16S rRNA. Nisin resistance determinant gene was determined by PCR amplification, enzyme digestion and sequencing.</p><p><b>RESULTS</b>Thirty nisin-resistant Lactococcus lactis strains from fresh milk samples were obtained. Three of these strains contained NSR gene of about 1000 bp as determined by agarose gel electrophoresis and further confirmed by enzyme digestion and sequence analysis. The NSR gene was located on the plasmid of Lactococcus lactis.</p><p><b>CONCLUSION</b>Complete NSR gene, located on the bacterial plasmid, has been successfully isolated from nisin-resistant Lactococcus lactis strains from fresh milk.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plasmids
/
RNA, Ribosomal, 16S
/
Polymerase Chain Reaction
/
Lactococcus lactis
/
Drug Resistance, Bacterial
/
Milk
/
Genetics
/
Microbiology
/
Anti-Bacterial Agents
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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