Dopamine receptors oppositely regulate cocaine-induced transcription factor CREB activation / 南方医科大学学报
Journal of Southern Medical University
;
(12): 715-718, 2006.
Article
in English
| WPRIM
| ID: wpr-282934
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of dopamine receptors in the regulation of the activity of transcription factor cAMP response element-binding protein (CREB) after cocaine treatment.</p><p><b>METHODS</b>By using dopamine receptor antagonists SCH23390 and nafadotride, the activation of CREB by D1 and D3 dopamine receptors after cocaine treatment and role of extracellular signal-regulated kinase (ERK) in cocaine-induced CREB activation were examined by Western blotting, which was also employed for determination of the effect of SCH23390 and nafadotride on CREB activation.</p><p><b>RESULTS</b>D1 receptor antagonist could inhibit cocaine-induced CREB activation, while D3 receptor antagonist enhanced cocaine-induced CREB activation. Dopamine receptor antagonists SCH23390 and nafadotride did not induce CREB activation. SL327, a MEK inhibitor, inhibited cocaine-induced CREB activation.</p><p><b>CONCLUSION</b>D1 and D3 dopamine receptors can oppositely regulate CREB activation after cocaine treatment and this regulation depends on ERK signaling pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Pyrrolidines
/
Benzazepines
/
Signal Transduction
/
Blotting, Western
/
Receptors, Dopamine D1
/
Cocaine
/
Cyclic AMP Response Element-Binding Protein
/
Dopamine Uptake Inhibitors
Limits:
Animals
Language:
English
Journal:
Journal of Southern Medical University
Year:
2006
Type:
Article
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