In vitro study of cytotoxic T lymphocyte activation by antigen-loaded dendritic cells for killing of K562 cells / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of dendritic cells (DC) stimulated with K562 cell lysate in inducing specific cytotoxic T lymphocytes (CTL) against K562 cells in vitro.</p><p><b>METHODS</b>The DCs were derived from healthy human peripheral blood monocytes in the presence of granulocyte-macrophage colony-stimulating factor, interleukin (IL)-4 and tumor necrosis factor (TNF) alpha. The T cells were stimulated by DCs loaded with freeze-thawed K562 cells and T-cell cytotoxicities were measured by lactate dehydrogenase (LDH) assay.</p><p><b>RESULTS</b>The DCs could be successfully obtained from peripheral blood monocyte after the culture. Mixed lymphocyte reactions induced by the antigen-loaded DC were much stronger than those induced by human peripheral blood monocytes (P<0.05). At the effector to target ratio of 101 and 201, cytotoxicities against K562 cells by CTL derived from culture with the antigen-loaded DCs were the strongest (P<0.05).</p><p><b>CONCLUSION</b>CTL derived from DCs pulsed with K562 cell lysate show effective and specific cytotoxicity against K562 cells.</p>