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Effects of mecobalamin on Bax and Bcl-2 in neurons after peripheral nerve injury / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 841-843, 2015.
Article in Chinese | WPRIM | ID: wpr-283012
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of mecobalamin on the expression of apoptosis-related proteins, Bax and Bcl-2, in neurons after peripheral nerve injury, and to explore the role of neuron apoptosis in peripheral nerve regeneration after injury.</p><p><b>METHODS</b>Thirty healthy adult male wistar rats were randomly divided into sham-operation group, model group, and mecobalamin group, with 10 rats in each group. A rat model of left sciatic nerve semi-injury was established using forceps. Rats in the mecobalamin group were fed mecobalamin, while rats in the sham-operation group and model group were given the same dose of normal saline. The protein expression of Bax and Bcl-2 in neurons was measured at 14 days after operation. A semi-quantitative analysis of Bax and Bcl-2 proteins was performed by image analysis technology.</p><p><b>RESULTS</b>The model group had significantly increased Bax protein expression and significantly reduced Bcl-2 protein expression in spinal cord anterior horn motor neurons and ganglion sensory neurons compared with the sham-operation group (P<0.05). Compared with the model group and sham-operation group, the mecobalamin group had significantly reduced Bax protein expression and significantly increased Bcl-2 protein expression in spinal cord anterior horn motor neurons and ganglion sensory neurons (P<0.05).</p><p><b>CONCLUSION</b>Mecobalamin has anti-apoptotic effect, and it contributes to neurological function recovery possibly by inhibiting the death of injured neurons.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Sciatic Nerve / Spinal Cord / Vitamin B 12 / Rats, Wistar / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Cell Biology / Bcl-2-Associated X Protein Limits: Animals Language: Chinese Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Sciatic Nerve / Spinal Cord / Vitamin B 12 / Rats, Wistar / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Cell Biology / Bcl-2-Associated X Protein Limits: Animals Language: Chinese Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases Year: 2015 Type: Article