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Effect of total flavonoids from astragalus complanatus on paraquat poisoning-induced pulmonary fibrosis in rats and its mechanisms / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 838-840, 2015.
Article in Chinese | WPRIM | ID: wpr-283013
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of total flavonoids from astragalus complanatus (FAC) on paraquat poisoning-induced pulmonary fibrosis in rats.</p><p><b>METHODS</b>The rats were divided into six groups randomly control group, paraquat group, prednisolone group and FAC low-dose, middle-dose, high-dose group. Pulmonary fibrosis model was replicated by intratracheal injection of paraquat. In the mext day,the rats were treated by intragastric administration once a day. After 28 days, the rats were sacrificed. The lung index and the levels of HYP and T-AOC were measured, and the pathologic changes of the lung tissue were obtained by HE staining. The levels of TGF-β, Smad2, α-SMA protein were analyzed by Western blot.</p><p><b>RESULTS</b>FAC improved the activity of T-AOC in serum and reduced pulmonary index and the content of HYP as well (P<0.05 or P<0.01), the alveolitis and fibrosis extent were attenuated. The expression of Smad2 significantly decreased in groups of FAC low-dose, middle-dose and high-dose (0.31±0.11, 0.45±0.12 and 0.30±0.05) as compared with that of the PQ group (0.85±0.34) (P<0.05). The expression of α-SMA significantly decreased in groups of FAC low-dose, middle-dose and high-dose (0.31±0.11, 0.35±0.07 and 0.32±0.10) as compared with that of the PQ group (0.45±0.08) (P<0.05). The expression of TGF-β significantly decreased in groups of FAC low-dose, middle-dose and high-dose (0.35±0.04, 0.27±0.05 and 0.18±0.04)as compared with that of the PQ group (0.63±0.11) (P<0.05).</p><p><b>CONCLUSION</b>FAC can alleviate PQ-induced pulmonary fibrosis in rats through inhibiting TGF-β/Smad signaling pathway.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Paraquat / Pathology / Pharmacology / Poisoning / Pulmonary Fibrosis / Flavonoids / Chemistry / Transforming Growth Factor beta / Actins / Astragalus Plant Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Paraquat / Pathology / Pharmacology / Poisoning / Pulmonary Fibrosis / Flavonoids / Chemistry / Transforming Growth Factor beta / Actins / Astragalus Plant Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases Year: 2015 Type: Article