Effects of specific small interfering RNA on Smoothened expression and LoVo cell proliferation and apoptosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1414-1417, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-283117
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of specific small interfering RNA (siRNA) on Smoothened (Smo) gene expression and the proliferation and apoptosis of colorectal cancer LoVo cells.</p><p><b>METHODS</b>Three different siRNAs (siRNA-1, siRNA-2, and siRNA-3, respectively) were transfected into LoVo cells via cationic liposome, and the changes of Smo mRNA level were determined using semi-quantitative RT-PCR 48 h after transfection. Flow cytometry and MTT assay were performed to assess the effect of the siRNAs on the proliferation and apoptosis of LoVo cells.</p><p><b>RESULTS</b>Forty-eight hours after Smo siRNA-1 transfection, Smo mRNA expression in LoVo cells decreased by about 63.56%, a reduction significantly greater than that in cells transfected with the other two siRNAs. The cell proliferation decreased significantly after Smo siRNA-1 transfection in comparison with the control cells, and 48 h after transfection, significantly higher apoptosis rate was observed in Smo siRNA-1-transfected cells than in the control cells.</p><p><b>CONCLUSION</b>Specific siRNA can significantly decrease Smo mRNA expression and inhibit the proliferation while inducing apoptosis of LoVo cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Transfection
/
Gene Expression Regulation, Neoplastic
/
Apoptosis
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Receptors, G-Protein-Coupled
/
Cell Proliferation
/
Smoothened Receptor
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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