Dynamic analysis of HBV cccDNA in HepG2 cells infected with Ad-1.2 HBV / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1374-1375, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-283126
ABSTRACT
<p><b>OBJECTIVE</b>o study the replication of hepatitis B virus (HBV) in HepG2 cells infected with Ad-1.2 HBV.</p><p><b>METHODS</b>HepG2 cells were transfected with adenovirus containing 1.2 copies of HBV DNA. The expression of HBV antigens were detected in the culture medium by means of enzyme-linked immunosorbent assay (ELISA), and the covalently closed circular DNA (cccDNA) in the cells was extracted with plasmid extraction kit and detected by real-time PCR with selective primer after treatment with mung bean nuclease.</p><p><b>RESULTS</b>HBsAg, HBeAg and HBV cccDNA were all detected in HepG2 cells after tranfection with Ad-1.2 HBV. HBV cccDNA was detected 1 day after the infection, reaching the peak level 4 days after infection.</p><p><b>CONCLUSION</b>Ad-1.2 HBV-infected cells can serve as the model for screening and evaluation of antiviral agents.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Time Factors
/
Virus Replication
/
Calibration
/
DNA, Viral
/
Transfection
/
Adenoviridae
/
Hepatitis B virus
/
Polymerase Chain Reaction
/
DNA, Complementary
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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