Effects of hepatic growth factor on c-kit+Lin- cell proliferation in mouse bone marrow / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1335-1337, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-283137
ABSTRACT
<p><b>OBJECTIVE</b>To determine the optimal cytokine combinations with hepatic growth factor (HGF) that results in the most significant simultaneous in vitro expansion of cc-kit(+)Lin(-) cells derived from the bone marrow.</p><p><b>METHODS</b>C-kit(+)Lin(-) cells were isolated from mouse bone marrow using a high-gradient magnetic cell sorting system (MACS) and expanded in the presence of stem cell factor (SCF), FLt-3 ligand (FL), leukemia inhibitor factor (LIF) thrombopoietin (TPO) and different concentrations of HGF for 7days in a liquid culture system. The total cell number and Annexin-V-positive cell number were counted, and the antigen expressions were studied with fluorescence-activated cell sorting (FACS).</p><p><b>RESULTS</b>In each group, c-kit(+)Lin(-) cells were expanded effectively and rapidly by 2 to 8 folds. Addition of 10 ng/ml HGF into SCF+FL+LIF+TPO resulted in the most significant expansion of c-kit(+)Lin(-) and total cells by 8.00 and 45.43 folds, respectively, with cell apoptosis rate of 17.42 %. But as the concentration of HGF increased, the c-kit(+)Lin(-) cells and the apoptosis rate decreased.</p><p><b>CONCLUSION</b>HGF at10 ng/ml shows optimal synergistic effect with SCF, FL, LIF and TPO in expansion of c-kit(+)Lin(-) cells, and excessive HGF may induce cell differentiation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bone Marrow Cells
/
Cell Count
/
Cell Differentiation
/
Hepatocyte Growth Factor
/
Proto-Oncogene Proteins c-kit
/
Cell Biology
/
Cell Proliferation
/
Dose-Response Relationship, Drug
/
Flow Cytometry
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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