Expression of DNA damage checkpoint mediator 1 and p53-binding protein 1 in human esophageal cancer cell lines TE-1, TE-13, and Eca109 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1314-1317, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-283142
ABSTRACT
<p><b>OBJECTIVE</b>To observe the expression of DNA damage checkpoint mediator 1 (MDC1) and p53-binding protein 1 (53BP1) at both mRNA and protein levels and their significance in different human esophageal cancer cell lines.</p><p><b>METHODS</b>In 3 human esophageal carcinoma cell lines, TE-1, TE-13 and Eca109 cells, the expressions of MDC1 and 53BP1 mRNA were detected with RT-PCR, and MDC1 and 53BP1 protein expressions were measured with immunohistochemistry, indirect immunofluorescence and Western blotting, respectively.</p><p><b>RESULTS AND CONCLUSIONS</b>MDC1 and 53BP1 expressions were observed for the first time in human esophageal carcinoma cell lines TE-1,TE-13 and Eca109 cells, at both the mRNA and protein levels. The expressions of MDC1 and 53BP1 proteins may be implicated in the radiosensitivity of human esophageal carcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
DNA Damage
/
RNA, Messenger
/
Esophageal Neoplasms
/
Immunohistochemistry
/
Nuclear Proteins
/
Gene Expression Regulation, Neoplastic
/
Trans-Activators
/
Blotting, Western
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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