Simultaneous determination of panaxynol and panaxydol in fibrous root of Panax ginseng by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish an HPLC method for simultaneous determination of panaxynol and panaxydol from the fibrous root of Panax ginseng.</p><p><b>METHOD</b>The analysis was performed on Elite C18 column (4.6 mm x 150 mm, 5 microm) with mobile phase gradient of CH3CN-water at a flow rate of 1.0 mL x min(-1). The detection wavelength was 230 nm, and the detection temperature was ambient.</p><p><b>RESULT</b>The linear range were 0.70-3.50 microg (r = 0.9995) for panaxynol, and 0.64-3.20 microg (r = 0.9999) for panaxydol. The average recoveries were 99.1% (RSD 1.7%) and 99.3% (RSD 1.2%), respectively.</p><p><b>CONCLUSION</b>The HPLC method is simple, rapid and reproducible, which can be used for the quality control of the fibrous root of P. ginseng.</p>