Simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-fLD after immunoaffinity column with online post-column photochemical derivatization / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2342-2346, 2011.
Article
in Zh
| WPRIM
| ID: wpr-283198
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for the simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-FLD after immunoaffinity column with online post-column photochemical derivatization.</p><p><b>METHOD</b>Sample was extracted with MeOH: H2O (80:20) and cleaned up by immunoaffinity column. The toxins were separated by reversed-phase HPLC and the mobile phase was consisted of methanol and 0.5% acetic acid solution with gradient elution. The determination was carried out by fluorescence detector after photochemical derivatization.</p><p><b>RESULT</b>The detection limits of aflatoxin G2, G1, B2, B1 and ochratoxin A were 0.02, 0.06, 0.015, 0.03 and 0.25 microg x kg(-1), respectively. The recoveries of analytes were from 76.0% to 103% and the relative standard deviations (RSDs) were below 13%.</p><p><b>CONCLUSION</b>The method is a simple, accurate and can be used to determine the contents of aflatoxin B1, B2, G1, G2 and ochratoxin A in G. uralensis simultaneously.</p>
Full text:
1
Index:
WPRIM
Main subject:
Chemistry
/
Chromatography, Affinity
/
Chromatography, High Pressure Liquid
/
Aflatoxins
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Glycyrrhiza uralensis
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Photochemical Processes
/
Methods
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Ochratoxins
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article