Your browser doesn't support javascript.
loading
Determination of underivatized glycine and proline in vinegar turtle shell by HPLC-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2107-2109, 2011.
Article in Chinese | WPRIM | ID: wpr-283247
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.</p><p><b>METHOD</b>An HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.</p><p><b>RESULT</b>The calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.</p><p><b>CONCLUSION</b>Since this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Quality Control / Reference Standards / Turtles / Proline / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Animal Shells / Glycine Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2011 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Quality Control / Reference Standards / Turtles / Proline / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Animal Shells / Glycine Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2011 Type: Article