Determination of underivatized glycine and proline in vinegar turtle shell by HPLC-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2107-2109, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-283247
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.</p><p><b>METHOD</b>An HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.</p><p><b>RESULT</b>The calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.</p><p><b>CONCLUSION</b>Since this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quality Control
/
Reference Standards
/
Turtles
/
Proline
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Animal Shells
/
Glycine
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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