Study on the effects of mitochondrial pathways on apoptosis in colon carcinoma cells induced by tumor necrosis factor related apoptosis inducing ligand / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 519-522, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-283282
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of mitochondrial pathways on apoptosis in colon carcinoma cells induced by Tumor necrosis factor related apoptosis inducing ligand and offer evidences for TRAIL application in clinic.</p><p><b>METHODS</b>Apoptosis, integration of mitochondria (including DeltaPsim, cardiolipin), activity of Caspase-9 and release of cytochrome c in colon carcinoma cells SW1116 treated with TRAIL, were detected by means of flowcytometry, fluorometer method and western-blot at the different time point.</p><p><b>RESULTS</b>After treated with TRAIL for 4 hours, the apoptosis index was 32.98%, and the damage of mitochondria occurred with DeltaPsim, cardiolipin decreased, and the activity of Caspase-9 and cytochrome c increased. The Caspase-9 activity at 24 hour was (48.12+/-2.21)micromol.L(-1).h(-1).mg(-1) protein. Mitochondrial damage induced by TRAIL could be inhibited by Caspase inhibitor Z-VAD. fmk.</p><p><b>CONCLUSION</b>Mitochondrial pathways involved in the apoptosis of colon carcinoma cell induced by TRAIL. Cytochrome c was released and Caspase-9 was activated in the Caspase-dependent manner after the damage of mitochondrial.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cardiolipins
/
Apoptosis
/
Cell Line, Tumor
/
Cytochromes c
/
Mitochondrial Membranes
/
Caspase 9
/
TNF-Related Apoptosis-Inducing Ligand
/
Receptors, TNF-Related Apoptosis-Inducing Ligand
/
Metabolism
/
Mitochondria
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2006
Type:
Article
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