Immunotherapeutic efficacy of both helper T lymphocytes and cytotoxic T lymphocytes epitopes augmented dendritic cells tumor vaccine on gastric cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 148-151, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-283363
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the immunotherapy efficacy of both helper T lymphocytes (Th) and cytotoxic T lymphocytes (CTL) epitopes augmented dendritic cells (DCs) tumor vaccine on gastric cancer.</p><p><b>METHODS</b>Naïve spleen T cells were stimulated by mixed peptides (a mixture of Th epitope MAGE-3 (22-36)) primed DCs per week in vitro. After 4 cycles of restimulation, peptide specific T cells were harvested and subgroups of which were determined with flow cytometry. Cytokines secreting profiles by CD4+ T cells and cytotoxicities of CD8+ T cells on tumor cells were assessed. The protective immunity by referred DCs tumor vaccines was also monitored.</p><p><b>RESULTS</b>Both Th and CTL epitopes primed DCs could elicit both CD4+ T cells and CD8+ T cells in vitro,of which CD4+ T cells released high amount of Th1 type cytokines (IFN-gamma, IL-2) on recognizing specific antigen, as well as CD8+ T cells exhibited efficient tumor-killing capacity. The effects induced by DCs pulsed with single epitope (Th or CTL epitope) in vivo were less effective than those induced by DCs pulsed with mixture epitopes.</p><p><b>CONCLUSIONS</b>Both Th and CTL epitopes augmented DCs tumor vaccine can induce CD4+ Th1 and CD8+ CTL mediated immune responses to eradicate gastric cancer cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Stomach Neoplasms
/
Therapeutics
/
Dendritic Cells
/
Melanoma, Experimental
/
T-Lymphocytes, Cytotoxic
/
Cell Line
/
T-Lymphocytes, Helper-Inducer
/
Epitopes, T-Lymphocyte
/
Cancer Vaccines
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2006
Type:
Article
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