Studies on preparation of recombinant hirudin-2 liposome and its pharmacokinetics by nasal delivery in rats / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 801-804, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-283383
ABSTRACT
<p><b>OBJECTIVE</b>To promote the nasal absorption of recombinant hirudin-2, the preparation and physicochemical properties of recombinant hirudin-2 liposomes, as well as its pharmacokinetic characteristics and bioavailability in rats after nasal administration were investigated.</p><p><b>METHOD</b>Recombinant hirudin-2 liposomes were prepared by reversal phase evaporation; the test of physicochemical properties including encapsulation efficiency, particle size and stability of liposome suspensions were determined by HPLC; Recombinant hirudin-2 concentration in plasma was determined by chromogenic substrate method and the relative bioavailability and pharmacokinetic parameters were also calculated using software program 3p87.</p><p><b>RESULT</b>The encapsulation efficiency of recombinant hirudin-2 liposome reached greater than 76.95%, with an average particle size of about 168.3 nm, size distribution ranging from 24 to 286 nm, relative peak width of +/- 0.47, and a good stability.</p><p><b>CONCLUSION</b>Compared with recombinant hirudin-2 solution, liposome preparation enhanced the nasal absorption of recombinant hirudin-2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Particle Size
/
Recombinant Proteins
/
Administration, Intranasal
/
Drug Carriers
/
Pharmacokinetics
/
Biological Availability
/
Chemistry
/
Hirudins
/
Technology, Pharmaceutical
/
Rats, Sprague-Dawley
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article
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