PCR, clone and sequence analysis of rDNA-ITS of Nelumbo nucifera from different geographical origins in China / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 671-675, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-283412
ABSTRACT
<p><b>OBJECTIVE</b>To provide DNA molecular marker for identification of Nelumbo nucifera by exploring the differences of nrDNA-ITS sequence of N. nucifera originated from different habitats.</p><p><b>METHOD</b>To compare nrDNA-ITS base sequence using specific PCR-ITS.</p><p><b>RESULT</b>The completed sequence of ITS and 5.8 S rDNA, and the partial sequences of 18S rDNA and 26S rDNA, totally 750 bp, from N. nucifera were obtained. The differences among N. nucifera from different habitats and from different cultivars were found.</p><p><b>CONCLUSION</b>The method can be used to identify N. nucifera among different species and to distinguish their fakes. It provided the basis for identifying N. nucifera from different geographical regions by comparison of their ITS sequences.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Plants, Medicinal
/
Species Specificity
/
RNA, Ribosomal
/
RNA, Ribosomal, 18S
/
Base Sequence
/
Deoxyribonuclease EcoRI
/
Deoxyribonucleases, Type II Site-Specific
/
Chemistry
/
China
Type of study:
Prognostic study
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article
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