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Study on apoptosis effect induced by isothiocyanates in broccoli on HepG-2 cells and its mechanism / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 612-615, 2007.
Article in Chinese | WPRIM | ID: wpr-283421
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis effect of isothiocyanates (ITCS) on human liver cancer cells HepG-2, and its mechanism.</p><p><b>METHOD</b>HepG-2 cells were treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test ROS levels, intracellular mitochondrial transmembrane potential (deltapsim) , and hypodiploid apoptosis peak in HepG-2 cells.</p><p><b>RESULT</b>ITCS obviously inhibited proliferation of HepG-2 cells. When treated with 15, 30, 60, 120, 240 microg x mL(-1) of ITCS for 24 h, ROS levels were (23.1+/-1. 8)%, (53.3+/-3.3)%, (57.9+/-2.0)%, (79.9+/-3.4)%, (93.4+/-2. 6)% respectively; and deltapsim were (94.8+/-5.5)%, (91.8+/-5.4)%, (66.0+/-5.6)%, (65. 5+/-6.6)% and (44.3+/-2.7)% respectively; when treated with 60, 120, 240 microg x mL(-1) of ITCS for 48 h, cell apoptotic rates were (16.6+/-2.8)%, (21.9+/-4.4) % and (70.2+/-5.3) % respectively.</p><p><b>CONCLUSION</b>ITCS generates ROS in gastric cancer HepG-2 cells, which causes mitochondrial membrane permeabilization and deltapsim decrease, therefore, leads to apoptosis of HepG-2 cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Physiology / Plants, Medicinal / Brassica / Mitochondria, Liver / Chemistry / Reactive Oxygen Species / Apoptosis / Isothiocyanates Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Physiology / Plants, Medicinal / Brassica / Mitochondria, Liver / Chemistry / Reactive Oxygen Species / Apoptosis / Isothiocyanates Limits: Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2007 Type: Article