Determination of butylidenephthalide in Ligusticum chuanxiong by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 508-510, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-283444
ABSTRACT
<p><b>OBJECTIVE</b>To determine butylidenephthalide in Ligusticum Chuanxiong with RP-HPLC.</p><p><b>METHOD</b>The sample was extracted with methanol using sonication. The ESTD was used to quantify butylidenephthalide. HPLC separation was carried out in a Hypersil ODS columm (4.6 mm x 150 mm, 5 microm) , eluted at 1 mL x min(-1) with methanol-5% isopropyl alcohol (60 40) at 25 degrees C. The detection wavelength was 230 nm.</p><p><b>RESULT</b>The linear range was 0.07-0.7 microg for butylidenephthalide. The average recovery was 95.3%, and RSD was 2.3% (n =6).</p><p><b>CONCLUSION</b>This method was simple and could be used to determine butylidenephthalide with satisfactory accuracy and reproducibility.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phthalic Anhydrides
/
Plants, Medicinal
/
Scattering, Radiation
/
Chemistry
/
China
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Ligusticum
/
Rhizome
/
Light
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article
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