Determination of aisaticoside in Centella asiatica extract by HPLC-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 503-505, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-283446
ABSTRACT
<p><b>OBJECTIVE</b>A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract.</p><p><b>METHOD</b>The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1).</p><p><b>RESULT</b>The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%).</p><p><b>CONCLUSION</b>The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Reference Standards
/
Scattering, Radiation
/
Triterpenes
/
Drugs, Chinese Herbal
/
Molecular Structure
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article
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