Detection of IgH gene rearrangement in B-cell non-Hodgkin's lymphoma and application of gel-scan method / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 513-517, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-283483
ABSTRACT
<p><b>OBJECTIVE</b>To develop a protocol for gene rearrangement study in non-Hodgkin's lymphoma (NHL) by PCR-directed gel-scan method and to set up quantitative criteria for IgH gene rearrangement which can be applied in the follow up of lymphoma patients.</p><p><b>METHODS</b>IgH gene rearrangement studies were carried out in 96 cases of B-cell NHL. The detection rate of clonality was evaluated. Sixty-five cases of IgH gene rearranged cases proven by FR3A-directed PCR and PAGE and 8 cases of benign lymphoid tissues (5 cases of reactive lymphoid hyperplasia, 3 cases of chronic tonsillitis), 5 cases of normal peripheral blood mononuclear cells were analyzed by gel-scan method and the proportion of h1/h2 (heights of peak1 and peak2 of gel-scan) was calculated.</p><p><b>RESULTS</b>The detection rate of IgH gene clonality was up to 68% using primer FR3A in the 96 B-cell NHL cases. The detection rate was up to 61% using primer FR2A. With a combination of primers FR3A and FR2A, the detection rate increased to 83%. Gel-scan curve showed that the value of h1/h2 was greater than 3 in all the 65 cases with IgH gene rearranged. In the 8 benign lymphoid tissue cases showed h1/h2 < 1.5, 5 cases with normal peripheral blood mononuclear cells showed a bell-shaped curve.</p><p><b>CONCLUSIONS</b>In the gel-scan curve of gene rearrangement studies in non-Hodgkin's lymphoma samples, the value of h1/h2 greater than 3 represents a true clonal proliferation. The peaks with relative heights less than 1.5 may not be significant and likely represent polyclonal cell population. A value between 1.5 and 3 however requires clinical follow-up. The success rate of rearrangement studies in B-cell NHL can be increased by using a combination of primers FR3A and FR2A.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Genes, Immunoglobulin
/
Electrophoresis, Gel, Two-Dimensional
/
Gene Rearrangement, B-Lymphocyte, Heavy Chain
/
Polymerase Chain Reaction
/
Lymphoma, B-Cell
/
Lymphoma, Large B-Cell, Diffuse
/
Immunoglobulin Heavy Chains
/
DNA Primers
/
Lymphoma, B-Cell, Marginal Zone
Type of study:
Diagnostic study
/
Practice guideline
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS