Pathological diagnosis and ultrastructure features of primary pulmonary cryptococcosis: a study of 27 cases / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 424-428, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-283498
ABSTRACT
<p><b>OBJECTIVE</b>To investigate diagnostic histopathology and ultrastructure features of primary pulmonary cryptococcosis (PC).</p><p><b>METHODS</b>Clinical data and pathologic findings of 27 cases of PC were retrospectively reviewed, light and electron microscopic evaluations and histochemistry stain studies were performed.</p><p><b>RESULTS</b>The specimens consisted of 2 fine-needle aspiration lung biopsies and 25 cases of open lung biopsies. Cryptococcosis granuloma formation was identifiable by histopathological examination in 25 of 27 cases, with gum-like lesion and fungi in the remaining 2 cases. The detection rates of cryptococcus neoformans (CN) by mucicarmine (MC), periodic acid-Schiff (PAS), alcian blue (AB) and Grocott methenamine-silver (GMS) were 87.0% (20/23), 100% (27/27), 66.7% (18/27), and 100% (23/23) respectively. Under the electron microscope, most CN had a simple structure with a few organelles. The capsule was seen in all organisms. A percentage of the organisms showed nuclei, nucleoli, mitochondria and vacuoles. The detection rate of CN by EM was 91.7% (11/12).</p><p><b>CONCLUSIONS</b>The clinical manifestation and imaging of PC are nonspecific for PC. Lung biopsy is the major diagnostic modality. The detection rate by electron microscopy was quite high. Therefore, a correct diagnosis of pulmonary cyrptococcosis should rely on the combination of histopathological evaluation, histochemistry staining and/or electron microscopic examination.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Microscopy, Electron
/
Retrospective Studies
/
Follow-Up Studies
/
Classification
/
Cryptococcosis
/
Cryptococcus neoformans
/
Biopsy, Fine-Needle
/
Diagnosis, Differential
/
Lung
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2004
Type:
Article
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