The role of Egr-1 and NF-kappaB in the pathogenesis of silicosis: an in-vitro study / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 363-367, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-283506
ABSTRACT
<p><b>OBJECTIVE</b>To study the correlation between the expression of Egr-1 and NF-kappaB and the up-regulation of TNF-alpha and TGF-beta1 in macrophages after stimulation by silica in-vitro.</p><p><b>METHODS</b>Macrophages were treated with antibodies against Egr-1 and NF-kappaB and antisense oligonucleotides. The level of TNF-alpha protein in the cell supernatant was then measured using enzyme-linked immunoadsorbent assay (ELISA). The expression of TGF-beta1 protein was detected by immunocytochemistry. The expression of TNF-alpha and TGF-beta1 mRNAs was also monitored by reverse transcriptase-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Compared with silica-stimulated macrophages untreated with antibodies, the cells treated with 10 micro g/ml of Egr-1 or NF-kappaB antibodies were associated with reduced expression of TNF-alpha and TGF-beta1 proteins and mRNAs (P < 0.05). Compared with silica-stimulated untransfected group, the antisense group was associated with obvious reduction in the expression of TNF-alpha and TGF-beta1 proteins and mRNAs (P < 0.05).</p><p><b>CONCLUSION</b>The expression of TNF-alpha and TGF-beta1 mRNAs and proteins are associated with activation of Egr-1 and NF-kappaB in macrophages, after stimulation by silica. It is possible that the corresponding antibodies and antisense oligonucleotides may become a potential therapeutic tool in the management of silicosis in the future.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Silicosis
/
Transcription Factors
/
RNA, Messenger
/
Cells, Cultured
/
NF-kappa B
/
Oligonucleotides, Antisense
/
Transforming Growth Factor beta
/
Tumor Necrosis Factor-alpha
/
Immediate-Early Proteins
Type of study:
Etiology study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2004
Type:
Article
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