Expression and significance of DARPP-32 in gastric carcinoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 350-353, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-283509
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the significance of DARPP-32 protein expression in gastric carcinoma tissue and cell lines.</p><p><b>METHODS</b>The expression of DARPP-32 protein in normal gastric mucosa and gastric carcinoma tissue was evaluated by immunohistochemical staining using streptavidin-biotin complex technique. The expression in gastric carcinoma tissue and cell lines was evaluated by Western blotting.</p><p><b>RESULTS</b>The expression rate of DARPP-32 protein in gastric adenocarcinoma tissue (92.7%) was significantly higher than that in normal gastric mucosa (52.6%, P < 0.05). There was no significant association between DARPP-32 protein expression and degree of tumor differentiation, local invasion and distant metastasis. As compared with adjacent non-carcinomatous gastric mucosa, both DARPP-32 and its truncated isoform t-DARPP were overexpressed in gastric adenocarcinoma tissue (t = 2.45, P = 0.015); and t-DARPP overexpression was more frequently seen. Expression of DARPP-32 and t-DARPP could also be detected in human gastric cancer cell lines. The expression of DARPP-32 protein was obviously reduced in SGC7901 drug-resistant cell strains.</p><p><b>CONCLUSIONS</b>DARPP-32 is overexpressed in gastric carcinoma. It may play an important role in gastric carcinogenesis. The underlying signal pathways in neoplastic gastric epithelium may also be related to the multi-drug resistance property of gastric cancer cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphoproteins
/
Stomach Neoplasms
/
Vincristine
/
Adenocarcinoma
/
Doxorubicin
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Cell Line, Tumor
/
Dopamine and cAMP-Regulated Phosphoprotein 32
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2004
Type:
Article
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