DNA sequence analysis for the promoter of pyruvate oxidase gene from Streptococcus oralis / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To elucidate the molecular structure of pyruvate oxidase gene promoter.</p><p><b>METHODS</b>The 1.30 kb fragment with promoter activity, amplified from upstream of Streptococcus oralis pyruvate oxidase gene (Sopox), was cloned into vector PBK-CMV. The positive transformed E. coli JM109 clone was selected, the recombinant plasmid was further identified with restriction mapping analysis. The positive recombinant plasmid was studied with sequence analysis.</p><p><b>RESULTS</b>After digesting the recombinant plasmid with Hind III, 1% agarose electrophoresis showed 1.30 kb fragment, which was consistent with predicted size. Sequence analysis revealed 1,350 bp.</p><p><b>CONCLUSION</b>The Sopox promoter region is sequenced. Further characterization of the Sopox promoter region will elucidate the molecular mechanism of H2O2 production of streptococcus oralis.</p>