Construction of the eukaryotic expression vector PsecTaq2A-AMG for human amelogenin / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this study was to construct a eukaryotic expression vector for human amelogenin (AMG).</p><p><b>METHODS</b>PCR was performed to amplify the AMG encoding region. Amplified fragments for human AMG were recovered and inserted into eukaryotic expression vectors PsecTaq2A. The recombinant plasmid PsecTaq2A-AMG was constructed and their positive clones were identified.</p><p><b>RESULTS</b>1. Amplified products were checked by electrophoresis and the results were satisfactory. 2. The recombinant plasmid PsecTaq2A-AMG was analyzed by restriction endonuclease mapping and DNA sequencing. The results of sequencing were consistent with those from GenBank.</p><p><b>CONCLUSION</b>The recombinant plasmid PsecTaq2A-AMG was successfully constructed with properly inserted DNA sequence encoding mature amelogenin.</p>