Tissue-engineered calcium phosphate cement in rabbit femoral condylar bone defects / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1993-1998, 2012.
Article
in English
| WPRIM
| ID: wpr-283680
ABSTRACT
<p><b>BACKGROUND</b>Calcium phosphate cement (CPC) is a favorable bone-graft substitute, with excellent biocompatibility and osteoconductivity. However, its reduced osteoinductive ability may limit the utility of CPC. To increase its osteoinductive potential, this study aimed to prepare tissue-engineered CPC and evaluate its use in the repair of bone defects. The fate of transplanted seed cells in vivo was observed at the same time.</p><p><b>METHODS</b>Tissue-engineered CPC was prepared by seeding CPC with encapsulated bone mesenchymal stem cells (BMSCs) expressing recombinant human bone morphogenetic protein-2 (rhBMP-2) and green fluorescent protein (GFP). Tissue-engineered CPC and pure CPC were implanted into rabbit femoral condyle bone defects respectively. Twelve weeks later, radiographs, morphological observations, histomorphometrical evaluations, and in vivo tracing were performed.</p><p><b>RESULTS</b>The radiographs revealed better absorption and faster new bone formation for tissue-engineered CPC than pure CPC. Morphological and histomorphometrical evaluations indicated that tissue-engineered CPC separated into numerous small blocks, with active absorption and reconstruction noted, whereas the residual CPC area was larger in the group treated with pure CPC. In the tissue-engineered CPC group, in vivo tracing revealed numerous cells expressing both GFP and rhBMP-2 that were distributed in the medullar cavity and on the surface of bony trabeculae.</p><p><b>CONCLUSION</b>Tissue-engineered CPC can effectively repair bone defects, with allogenic seeded cells able to grow and differentiate in vivo after transplantation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
General Surgery
/
Bone Cements
/
Recombinant Proteins
/
Calcium Phosphates
/
Cells, Cultured
/
Chemistry
/
Transforming Growth Factor beta
/
Tissue Engineering
/
Bone Morphogenetic Protein 2
/
Femur
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2012
Type:
Article
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