Determination of paeoniflorin in rat plasma by HPLC-MS/MS and its pharmacokinetics / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>An HPLC-MS/MS assay was established to determine paeoniflorin in rat plasma and be used to investigate the effect of pharmacokinetics of paeoniflorin when co-administrated Radix Angelicae Sinensis and Radix Paeoniae Rubra.</p><p><b>METHOD</b>HPLC-MS/MS was performed in the multiple reaction monitoring (MRM) mode using target ions at m/z 450 --> m/z 327 for paeoniflorin and m/z 388 --> m/z 225 for jasminoidin. A single dose of Radix Paeoniae Rubra alone and with Radix Angelicae Sinensis was given to rats by ig, the dosage of paeoniflorin was 294.78 mg x kg(-1). Concentrations of paeoniflorin in rat plasma were determined by HPLC-MS/MS assay and main pharmacokinetic parameters were estimated.</p><p><b>RESULT</b>The mainly pharmacokinetic parameters of paeoniflorin when administrated Radix Paeoniae Rubra only were as follows C(max) (1.55 +/- 0.53) mg x L(-1), T(max) (0.9 +/- 0.3) h, t 1/2 (1.51 +/- 0.63) h, MRT (3.08 +/- 0.74) h, AUC(0 --> tau) (4.68 +/- 0.85) mg x h(-1) x L(-1). When the two drugs administrated together, the corresponding parameters were (0.93 +/- 0.42) mg x L(-1), (1.5 +/- 0.8) h, (3.08 +/- 1.79) h, (5.19 +/- 1.95) h, (3.36 +/- 0.56) mg x h(-1) x L(-1), respectively. The parameters of C(max), AUC(0 --> tau) and MRT were significantly changed (P < 0.05).</p><p><b>CONCLUSION</b>Radix Angelicae Sinensis can significantly affect the pharmacokinetics of paeoniflorin, the principal component of Radix Paeoniae Rubra.</p>