Analysis of principal composition of ethyl acetate part in Huangqi Danggui decoction by HPLC-ESI-TOF-MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2508-2512, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-283850
ABSTRACT
<p><b>OBJECTIVE</b>To analyze and identify the principal composition of ethyl acetate part of Huangqi Danggui decoction by high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS).</p><p><b>METHOD</b>The analysis conditions are as follows Zorbax SB C18 (4.6 mm x 250 mm, 5 microm) column; mobile phase (A) water mobile phase (B) acetonitrile, gradient elution; UV detection wavelength 254 nm; ESI source and data acquisition in positive and negative mode.</p><p><b>RESULT</b>The accurate molecular weights of 20 compounds were measured and identified in ethyl acetate part of Huangqi Danggui decoction. Furthermore, the types of them are as below flavonoids and flavonoid glycosides, saponins, oligosaccharides, amino acids and phthalides.</p><p><b>CONCLUSION</b>It is a rapid and accurate method that the compositions of compound prescription of traditional Chinese medicine can be identified in terms of the separation of high performance liquid chromatography, the accurate molecular weights measured by MS and other information, which can clarify the potential effective compounds of Huangqi Danggui decoction.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligosaccharides
/
Saponins
/
Benzofurans
/
Flavonoids
/
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Spectrometry, Mass, Electrospray Ionization
/
Amino Acids
/
Glycosides
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article
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