IL-1β promotes the hematopoietic support of human umbilical cord mesenchymal stem cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1005-1009, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-283992
ABSTRACT
This study was aimed to investigate the effect of IL-1β on hematopoietic support of human umbilical cord mesenchymal stem cells (hUC-MSC). 2×10(6) hUC-MSC were seeded in 75 cm(2) flasks, after adherence to wall for 2 h, 10 ng/ml IL-1β was added in hUC-MSC supernatant and cultured for 36 h, then the culture supernatants and cells were harvested. The effect of conditioned medium with/without IL-1β on CD34(+) cell hematopoietic support was observed, mRNA expression changes of hUC-MSC cultured in medium with/without IL-1β were monitored by real time PCR, the differences in hematopoiesis-related factors were detected by ELISA. The results showed that the conditioned culture medium of hUC-MSC with IL-1β enhanced the ability to form colony of CD34(+) cells, especially CFU-G and CFU-GM in vitro; IL-1β promoted the mRNA expression of GM-CSF, G-CSF, IL-6 on MSC; IL-1β also promoted the secretion of GM-CSF, G-CSF, and IL-6 protein from hUC-MSC. It is concluded that IL-1β enhances hematopoietic support capacity especially, capability of MSC to myeloid differentiation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Umbilical Cord
/
Cell Differentiation
/
Cells, Cultured
/
Granulocyte Colony-Stimulating Factor
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Interleukin-6
/
Bodily Secretions
/
Culture Media
/
Cell Biology
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article
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