Expression of blood Th17 and CD4(+) CD25(+) Treg cells in patients with aplastic anemia / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 974-978, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-283998
ABSTRACT
This study was aimed to investigate the expression of blood Th17 and CD4(+) CD25(+) regulatory T cells (Treg) in the patients with aplastic anemia (AA). Forty-five patients with AA were enrolled into this study, and were divided into mild aplastic anemia (MAA) group (n = 25) and severe aplastic anemia group (SAA) (n = 20), blood cell count was recorded. 15 healthy donors were enrolled as control. Proportions of blood Th17 and CD4(+) CD25(+) Treg cells were determined by flow cytometry. The serum levels of IL-17, IFN-γ and TNF-α, as well as their concentrations in culture supernatant of phytohemagglutinin (PHA) -stimulated peripheral blood mononuclear cells, were measured by ELISA. The results showed that the proportions of blood Th17 cells and concentration of blood serum IL-17 and IFN-γ increased in patients with SAA, compared with MAA and normal controls, but CD4(+) CD25(+) Foxp3(+) Treg cells obviously decreased in patients with SAA. The concentrations of IL-17 and IFN-γ significantly increased in culture supernatant of SAA group. Hemoglobin level in the patients with AA negatively correlated with the population of Th17 cells and serum IL-17 level, whereas positively correlated with the expression of CD4(+) CD25(+)Treg cells. It is concluded that the increased response of Th17 cells and deficiency of CD4(+) CD25(+) Treg cells present in severe aplastic anemia. The severity of anemia may be related with the imbalance between Th17 and CD4(+) CD25(+)Treg cell response.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Leukocytes, Mononuclear
/
Case-Control Studies
/
Interferon-gamma
/
Tumor Necrosis Factor-alpha
/
T-Lymphocytes, Regulatory
/
Interleukin-17
/
Th17 Cells
/
Anemia, Aplastic
/
Metabolism
Type of study:
Observational study
/
Risk factors
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article
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