beta-catenin/Tcf signaling in squamous differentiation of porcine airway epithelial cells / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 121-124, 2008.
Article
in English
| WPRIM
| ID: wpr-284629
ABSTRACT
For a preliminary study of the role of beta-catenin/Tcf signaling in squamous differentiation of airway (tracheobronchial) epithelial cells, a stable mutant of beta-catenin was transfected into primarily cultured porcine airway epithelial cells. Western blotting revealed that exogenous protein was observed in large quantity in cytoplasm and nucleus. When co-transfected with Tcf luciferase reporter plasmids, beta-catenin mutant increased the reporter's transcriptional activities. However, mRNA expression of a squamous differentiation marker, small proline-rich protein (SPRP), was not elevated, as shown by reverse transcription-polymerase chain reaction. These findings suggest that beta-catenin/Tcf signaling may not be directly involved in the squamous differentiation of porcine airway epithelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Trachea
/
Transcription, Genetic
/
RNA, Messenger
/
Signal Transduction
/
Cell Differentiation
/
Cell Nucleus
/
Cells, Cultured
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
Limits:
Animals
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2008
Type:
Article
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