Human ribonuclease 9, a member of ribonuclease A superfamily, specifically expressed in epididymis, is a novel sperm-binding protein / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 240-251, 2009.
Article
in English
| WPRIM
| ID: wpr-284700
ABSTRACT
To explore the functions of human ribonuclease 9 (RNase 9), we constructed a mammalian fusion expression vector pcDNA-hRNase9, prepared recombinant human RNase 9-His fusion protein from HEK293T cells and determined its N-terminal amino acid sequences. According to the determined mature protein, recombinant human RNase 9 was prepared in E. coli. Ribonucleolytic activity and antibacterial activity of recombinant human RNase 9 were detected, and the distribution of human RNase 9 on tissues and ejaculated spermatozoa and in vitro capacitated spermatozoa were analyzed via indirect immunofluorescence assay. The results showed that recombinant human RNase 9 did not exhibit detectable ribonucleolytic activity against yeast tRNA, but exhibited antibacterial activity, in a concentration/time dependent manner, against E. coli. Immunofluorescent analyses showed that the predicted human RNase 9 was present throughout the epididymis, but not present in other tissues examined, and human RNase 9 was also present on the entire head and neck regions of human ejaculated spermatozoa and in vitro capacitated spermatozoa. These results suggest that human RNase 9 may play roles in host defense of male reproductive tract.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ribonuclease, Pancreatic
/
Ribonucleases
/
Spermatozoa
/
Testis
/
Recombinant Fusion Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Molecular Sequence Data
/
Chemistry
/
Blotting, Western
/
Amino Acid Sequence
Limits:
Adult
/
Humans
/
Male
Language:
English
Journal:
Asian Journal of Andrology
Year:
2009
Type:
Article
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