Screening potential DNA barcode regions of genus Papaver / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2964-2969, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-284817
ABSTRACT
DNA barcoding is an effective technique in species identification. To determine the candidate sequences which can be used as DNA barcode to identify in Papaver genus, five potential sequences (ITS, matK, psbA-trnH, rbcL, trnL-trnF) were screened. 69 sequences were downloaded from Genbank, including 21 ITS sequences, 10 matK sequences, 8 psbA-trnH sequences, 14 rbcL sequences and 16 trnL-trnF sequences. Mega 6.0 was used to analysis the comparison of sequences. By the methods of calculating the distances in intraspecific and interspecific divergences, evaluating DNA barcoding gap and constructing NJ and UPMGA phylogenetic trees. The sequence trnL-trnF performed best. In conclusion, trnL-trnF can be considered as a novel DNA barcode in Papaver genus, other four sequences can be as combination barcode for identification.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papaver
/
Classification
/
DNA Barcoding, Taxonomic
/
Genetics
/
Methods
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article
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