Relationship between mutations of mitochondrial DNA ND1 gene and type 2 diabetes / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 985-989, 2004.
Article
in English
| WPRIM
| ID: wpr-284864
ABSTRACT
<p><b>BACKGROUND</b>Recent studies have indicated that many mutations in mitochondrial (mt) DNA NDI gene region are related to diabetes mellitus. In this study we explored the relationship between various mtDNA ND1 gene mutations and type 2 diabetes mellitus (DM) among Chinese.</p><p><b>METHODS</b>Using PCR restriction fragment length polymorphism (PCR-RFLP) analysis and gene sequencing, 4 spots of mtDNA (nt3243, nt3316, nt3394, nt3426) were screened in 478 diabetics and 430 non-diabetic subjects.</p><p><b>RESULTS</b>In diabetic group, there were 13 carriers (2.72%) of 3316 G-->A mutation,12 (2.51%) of 3394 T-->C mutation and 2 (0.42%) of 3426A-->G mutation. In controls, only 3394 T-->C mutation was observed in 2 subjects (0.47%). There was significant difference in the frequency of 3316 and 3394 mutation between two groups (P < 0.05, respectively). More subjects with mitochondrial DNA ND1 gene mutations had DM family history and greater tendency of maternal inheritance when compared to those patients without mutation in diabetic group (P < 0.01). A 3426 mutation diabetic pedigree was studied, and we found 12 maternal members in the family had the same mutation.</p><p><b>CONCLUSION</b>mtDNA ND1 gene mutations at nt3316 (G-->A), nt3394 (T-->C) and 3426 (A-->G) might contribute to the pathogenesis of DM with other genetic factors and environment factors.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pedigree
/
Polymorphism, Restriction Fragment Length
/
DNA, Mitochondrial
/
Molecular Sequence Data
/
Base Sequence
/
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
Diabetes Mellitus, Type 2
/
Genetics
/
Mutation
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2004
Type:
Article
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