Influence of maternal age on meiotic spindle and chromosome configuration of oocytes / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of maternal age on meiotic spindle and chromosome configuration of oocytes.</p><p><b>METHODS</b>Spindle and chromosome configuration was examined in day 1 unfertilized human oocytes after in vitro fertilization (IVF) and intracytoplasmic sperm injection(ICSI) by immunocytochemistry and visualized by laser confocal microscopy.</p><p><b>RESULTS</b>Statistically significant differences were observed on normal spindle and chromosome configurations of oocytes between 25-29 maternal age group (33% and 31%, respectively), and 30-34 age group (P< 0.05) as well as 35-40 age group(0%, P<0.01). The incidence of abnormal spindle and chromosome configurations of oocytes from 30-34 and 35-40 maternal age groups was much higher than that of oocytes from 25-29 age group (P<0.01, P<0.05).</p><p><b>CONCLUSION</b>Incidence of abnormal spindle and chromosome configuration of oocytes is related to maternal age. It could be an important reason of age related oocyte aneuploidy.</p>