Sperm sex chromosome analysis and preimplantation genetic diagnosis of patients with sex chromosome anomalies / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 431-433, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-285108
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the constitution of abnormal spermatozoa from patients with sex chromosome anomalies.</p><p><b>METHODS</b>Triple color fluorescence in situ hybridization (FISH) was used to determine the sex chromosome constitution of spermatozoa from three patients with sex chromosome anomalies (case 146,XY/47,XXY, case 245,XO/46,X,Yqh-, case 347,XXY). The preimplantation genetic diagnosis (PGD) was performed to case 2.</p><p><b>RESULTS</b>An increased ratio (2.05 vs 1) of X-bearing to Y-bearing spermatozoa was only observed in case 2, who also had an increased incidence of total abnormal spermatozoa (29.71%). An increased incidence of total abnormal spermatozoa (4.91%) was also observed in case 3. Among the constitution of abnormal spermatozoa, case 2 had the increased proportions of XY18 disomy, O18 monosomy and XO monosomy, while case 3 had an increase proportion of XY18 disomy (1.87%). PGD was performed to case 2 and one embryo with XX1818 was selected for implanting.</p><p><b>CONCLUSION</b>Using FISH to detect the sperm sex chromosomes in patients with sex chromosome anomalies can provide the useful information to evaluate the risk of sex chromosome anomalies in preimplantation embryos.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sex Chromosome Aberrations
/
Spermatozoa
/
In Situ Hybridization, Fluorescence
/
Preimplantation Diagnosis
/
Chromosomes, Human, X
/
Chromosomes, Human, Y
/
Genetics
/
Metabolism
/
Methods
Type of study:
Diagnostic study
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2006
Type:
Article
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