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Stereotactic injection of shrna GSK-3β-AAV promotes axonal regeneration after spinal cord injury / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 548-553, 2016.
Article in English | WPRIM | ID: wpr-285231
ABSTRACT
Evidence suggested that glycogen synthase kinase-3β (GSK-3β) is involved in Nogo-66 inhibiting axonal regeneration in vitro, but its effect in vivo was poorly understood. We showed that stereotactic injection of shRNA GSK-3β-adeno associated virus (GSK-3β-AAV) diminished syringomyelia and promoted axonal regeneration after spinal cord injury (SCI), using stereotactic injection of shRNA GSK-3β-AAV (tested with Western blotting and RT-PCR) into the sensorimotor cortex of rats with SCI and by the detection of biotin dextran amine (BDA)-labeled axonal regeneration. We also determined the right position to inject into the sensorimotor cortex. Our findings consolidate the hypothesis that downregulation of GSK-3β promotes axonal regeneration after SCI.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Spinal Cord Injuries / Axons / Syringomyelia / Therapeutics / Dependovirus / RNA, Small Interfering / Sensorimotor Cortex / Glycogen Synthase Kinase 3 beta / Genetics Limits: Animals / Humans Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Spinal Cord Injuries / Axons / Syringomyelia / Therapeutics / Dependovirus / RNA, Small Interfering / Sensorimotor Cortex / Glycogen Synthase Kinase 3 beta / Genetics Limits: Animals / Humans Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2016 Type: Article