Role of axl in preeclamptic EPCs functions / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 395-401, 2016.
Article
in English
| WPRIM
| ID: wpr-285256
ABSTRACT
Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells (EPCs). Axl was detected by RT-PCR and Western blotting in both placentas and EPCs from normal pregnancy and preeclampsia patients. The Axl inhibitor, BMS777-607, was used to inhibit the Axl signalling pathway in EPCs. Cell proliferation, differentiation, migration and adhesion were measured by CCK-8 assay, cell differentiation assay, Transwell assay, and cell adhesion assay, respectively. Results showed the expression levels of Axl mRNA and protein were significantly higher in both placentas and EPCs from preeclampsia patients than from normal pregnancy (P<0.05). After treatment with BMS777-607, proliferation, differentiation, migration and adhesion capability of EPCs were all significantly decreased. Our study suggests Axl may play a role in the function of EPCs, thereby involving in the pathogenesis of preeclampsia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Placenta
/
Pre-Eclampsia
/
Pyridones
/
Stem Cells
/
Blood
/
Blood Pressure
/
RNA, Messenger
/
Case-Control Studies
Type of study:
Observational study
Limits:
Adult
/
Female
/
Humans
/
Pregnancy
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2016
Type:
Article
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