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Neamine inhibits growth of pancreatic cancer cells in vitro and in vivo / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 82-87, 2016.
Article in English | WPRIM | ID: wpr-285307
ABSTRACT
Neamine, a non-toxic derivative of neomycin, has recently been shown to have antitumor activities in various types of cancers. However, its effect on pancreatic cancer is still unknown. The study aimed to investigate its antitumor activity on pancreatic cancer and the underlying mechanisms. MTT assay was used to observe the effect of neamine on angiogenin (ANG)-induced AsPC-1 cell proliferation. Tissue microassay and immunofluorescence staining were used to detect the expression of ANG and its nuclear translocation, respectively. Tumor xenografts were established by subcutaneous inoculation of AsPC-1 pancreatic cancer cells into the right flanks of nude mice, and neamine was injected subcutaneously. Immunohistochemistry was done to observe the expression of ANG, CD31 and Ki-67 in tumor xenografts. It was found that neamine blocked the nuclear translocation of ANG effectively and inhibited ANG-induced AsPC-1 cell proliferation in a dose-dependent manner. Neamine had anti-tumor effects on AsPC-1 xenograft models. Consistently, neamine reduced the expression levels of ANG, Ki-67 and CD31 in tumor xenografts. It was concluded that neamine may be a promising agent for treatment of pancreatic cancer.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pancreatic Neoplasms / Pharmacology / Ribonuclease, Pancreatic / Carcinoma / Framycetin / Platelet Endothelial Cell Adhesion Molecule-1 / Ki-67 Antigen / Cell Line, Tumor / Therapeutic Uses / Cell Proliferation Limits: Animals / Humans / Male Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pancreatic Neoplasms / Pharmacology / Ribonuclease, Pancreatic / Carcinoma / Framycetin / Platelet Endothelial Cell Adhesion Molecule-1 / Ki-67 Antigen / Cell Line, Tumor / Therapeutic Uses / Cell Proliferation Limits: Animals / Humans / Male Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2016 Type: Article