Study on sensitization and mechanism of CGA-BSA / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the sensitization and mechanism of artificial antigen of chlorogenic acid (CGA-BSA).</p><p><b>METHOD</b>Using intensive immunization to establish allergy animal model on guinea pig and preparing antiserum and tissue for further test. Using HE staining to observe pathology change of lungs, trachea, liver. Using passive mast cell (PMC) degranulation test to observe the immunogenicity of CGA-BSA and using ELISA to detect IgE and histamine in plasma.</p><p><b>RESULT</b>There established allergy animal model on guinea pig, which include a increase cell degranulation by a ratio (63.58 +/- 10.23)% in PMC test, increase of specific antibody IgE and increase of histamine in plasma after provocation by ELISA.</p><p><b>CONCLUSION</b>Allergen CGA-BSA could provoke allergenic response in guinea pig, and the allergic response belongs to type I allergy.</p>